Washington [US], January 10 (ANI): A new study has found that the air in a zoo has enough DNA to identify animals nearby, a finding that may prove to be a valuable, non-invasive tool to track biodiversity.
The research has been published in the 'Current Biology Journal'.
"Capturing airborne environmental DNA from vertebrates makes it possible for us to detect even animals that we cannot see are there," said researcher Kristine Bohmann and head of the team at the University of Copenhagen.
Terrestrial animals can be monitored in many ways: directly by the camera and in-person observation, or indirectly by what they leave behind, like footprints or faeces. The drawback to these methods is that they can involve intensive fieldwork and require the animal to be physically present. For example, monitoring animals by camera requires knowledge of where to put the cameras on the animal's path, sifting through thousands of pictures, and usually a bit of luck.
"Earlier in my career, I went to Madagascar hoping to see lots of lemurs. But in reality, I rarely saw them. Instead, I mostly just heard them jumping away through the canopy." said Bohmann.
"So, for many species, it can be a lot of work to detect them by direct observation, especially if they are elusive and live in very closed or inaccessible habitats," she added"Compared to what people find in rivers and lakes, monitoring airborne DNA is really, really hard, because the DNA seems super diluted in the air," said Elizabeth Clare, lead researcher of the Queen Mary University of London team (Clare is now at York University in Toronto).
"But our zoo studies have yet to fail for different samplers, genes, locations, and experimental approaches. All of it worked and surprisingly well," she added.
Bohmann and Clare draw heavily from their past research monitoring wildlife by collecting other sample types containing DNA shed by animals. This is referred to as "environmental DNA," or eDNA, and is a well-established technique used most frequently to monitor aquatic organisms by sequencing eDNA from water samples.
"Air surrounds everything, and we wanted to avoid contamination in our samples while optimizing true detection of animal DNA. Our newest work with airborne eDNA involves what we usually do when processing eDNA samples, just tuned up a little bit," said Bohmann.
Each research group conducted their study at a local zoo by collecting samples at various places in the zoo, including inside walled-in enclosures like the tropical house and indoor stables, as well as outdoor enclosures in the open air.
"To collect airborne eDNA, we used a fan, like the one you would use to cool down a computer and attach a filter to it. We then let it run for some time," said Christina Lynggaard, first author and postdoctoral fellow at the University of Copenhagen.
The fan draws in air from the zoo and its surroundings, which could contain genetic material from any number of sources, like breath, saliva, fur, or faeces, though the researchers have not determined the exact source.
"It could be anything that can become airborne and is small enough to continue floating in the air," said Lynggaard.
"After air filtration, we extracted the DNA from the filter and used PCR amplification to make a lot of copies of the animal DNA. After DNA sequencing, we processed the millions of sequences and ultimately compared them to a DNA reference database to identify the animal species," she added.
"There's a leap of faith component to some of this because when you deal with regular tissue or even aquatic DNA samples, you can measure how much DNA you have, but with these samples, we're dealing with forensically tiny amounts of DNA," said Clare.
"In many cases, when we only sample for a few minutes we can't measure the DNA, and so we have to jump to the next stage of PCR where we find out whether there's any in it or not. When we sample for hours we get more but there is a trade-off," she added.
In each study, the researchers detected animals inside the zoo and wildlife from nearby. Clare's team from the Queen Mary University of London detected DNA from 25 species of mammals and birds, and even DNA belonging to the Eurasian hedgehog, which is endangered in the UK.
Bohmann's team at the University of Copenhagen team detected 49 non-human vertebrate species, including mammal, bird, reptile, amphibian, and fish species. These included zoo animals like the okapi and armadillo and even the guppy in a pond in the tropical house, locally occurring animals like squirrels, and pest animals like the brown rat and house mouse. Further, they detected fish species used for feed for other animals in the zoo. Both teams took extensive measures to check that their samples were not contaminated, including by DNA already in their labs.
By choosing a zoo for the location of their studies, the researchers knew the position of a large collection of non-native species, so they could tell the difference between a real signal and a contaminant.
"We had originally thought of going to a farm, but if you pick up cow DNA you must ask 'Is that cow here or is it some cow a hundred miles away or in someone's lunch?'" said Clare.
"But by using the zoo as a model there's no other way I would detect DNA from a tiger, except for the zoo's tiger. It lets us really test the detection rate," she added.
"One thing both our labs do is develop and apply new tools, so perhaps it's not so surprising that we both ended up with the same idea at the same time," said Clare. (ANI)